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Proceedings Paper

Microfluidic system for in-vitro hypoxia assays
Author(s): M. Busek; S. Grünzner; T. Steege; C. Steinfelder; F. Schmieder; U. Klotzbach; F. Sonntag
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Paper Abstract

Hereby presented is a microfluidic system, including a micro pump, an oxygenator and a cell culture chamber for perfusion controlled hypoxia assays. It consists of laser-structured polycarbonate (PC) foils and an elastomeric membrane which were joined together using thermal diffusion bonding. The elastomer forms an oxygenator element. The microfluidic system is characterized using non-invasive flow measurement based on micro-Particle-ImageVelocimetry (μPIV) and optical oxygen measurement utilizing the oxygen dependent fluorescence decay. Based on those experimental results and mathematical considerations, the oxygenator and mass transport phenomena within the microfluidic system can be described. This oxygen sensor, the micro pump, a controlling device and the gas mixture at the oxygenator forms a regulatory circuit to adjust the oxygen content in the cell culture chamber and helps to produce well-defined hypoxic conditions for the cells.

Paper Details

Date Published: 28 February 2017
PDF: 10 pages
Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 1006110 (28 February 2017); doi: 10.1117/12.2253664
Show Author Affiliations
M. Busek, Fraunhofer IWS Dresden (Germany)
S. Grünzner, Fraunhofer IWS Dresden (Germany)
TU Dresden (Germany)
T. Steege, Fraunhofer IWS Dresden (Germany)
C. Steinfelder, Fraunhofer IWS Dresden (Germany)
F. Schmieder, Fraunhofer IWS Dresden (Germany)
U. Klotzbach, Fraunhofer IWS Dresden (Germany)
F. Sonntag, Fraunhofer IWS Dresden (Germany)


Published in SPIE Proceedings Vol. 10061:
Microfluidics, BioMEMS, and Medical Microsystems XV
Bonnie L. Gray; Holger Becker, Editor(s)

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