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Proceedings Paper

Assessment of geometry in 2D immune systems using high accuracy laser-based bioprinting techniques (Conference Presentation)
Author(s): Sara Lauzurica; Andrés Márquez; Carlos Molpeceres; Laura Notario; Miguel Gómez-Fontela; Pilar Lauzurica
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Paper Abstract

The immune system is a very complex system that comprises a network of genetic and signaling pathways subtending a network of interacting cells. The location of the cells in a network, along with the gene products they interact with, rules the behavior of the immune system. Therefore, there is a great interest in understanding properly the role of a cell in such networks to increase our knowledge of the immune system response. In order to acquire a better understanding of these processes, cell printing with high spatial resolution emerges as one of the promising approaches to organize cells in two and three-dimensional patterns to enable the study the geometry influence in these interactions. In particular, laser assisted bio-printing techniques using sub-nanosecond laser sources have better characteristics for application in this field, mainly due to its higher spatial resolution, cell viability percentage and process automation. This work presents laser assisted bio-printing of antigen-presenting cells (APCs) in two-dimensional geometries, placing cellular components on a matrix previously generated on demand, permitting to test the molecular interactions between APCs and lymphocytes; as well as the generation of two-dimensional structures designed ad hoc in order to study the mechanisms of mobilization of immune system cells. The use of laser assisted bio-printing, along with APCs and lymphocytes emulate the structure of different niches of the immune system so that we can analyse functional requirement of these interaction.

Paper Details

Date Published:
PDF: 1 pages
Proc. SPIE 10062, Optical Interactions with Tissue and Cells XXVIII, 100620Q; doi: 10.1117/12.2253622
Show Author Affiliations
Sara Lauzurica, Univ. Politécnica de Madrid (Spain)
Andrés Márquez, Univ. Politécnica de Madrid (Spain)
Carlos Molpeceres, Univ. Politécnica de Madrid (Spain)
Laura Notario, Ctr. Nacional de Microbiología, Instituto de Salud Carlos III (Spain)
Miguel Gómez-Fontela, Centro Nacional de Microbiología, Instituto de Salud Carlos III (Spain)
Univ. Politécnica de Madrid (Spain)
Pilar Lauzurica, Ctr. Nacional de Microbiología, Instituto de Salud Carlos III (Spain)
Univ. Politécnica de Madrid (Spain)

Published in SPIE Proceedings Vol. 10062:
Optical Interactions with Tissue and Cells XXVIII
E. Duco Jansen; Hope Thomas Beier, Editor(s)

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