Share Email Print
cover

Proceedings Paper

Ring resonator for biosensing via flow-through approach (Conference Presentation)
Author(s): Romeo Bernini; Immacolata Angelica Grimaldi; Gianluca Persichetti; Genni Testa
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The realization of a simple real time biosensor, in which antibodies are immobilized onto surfaces, represents a promising application in the immunoassay development. Among the various sensing approaches, one of the most promising is based on microring resonators, offering a lot of advantages such as mass production, reduced dimensions, label-free and real time detection. The use of the evanescent field as optical transduction principle allows the development of label-free biosensors, in which the antibody is usually immobilized on the sensor surface and the binding of the antigen can be controlled and followed in real-time. The overall performances of immunosensors are strongly related to the optimization of the immobilization process and the integration between the microfluidic parts and the optical detection system. The combination of these two aspects makes the biosensing process very efficient, with a consequent reduction of the response time and improvement of the immobilization process efficiency. In this work we explore the working mechanism of a flow-through microresonator platform. A drilled hole, in the center of the ring, allows the active transport mechanism of the analyte toward the sensing surface with a consequent reduction of the response time. Moreover, we study the effects of oxygen plasma, in terms of duration times and plasma power, on immobilization efficiency of immunoglobulin G (IgG). An improvement of about 20% of the protein adsorption is ascribed to chemico-physical modification of SU-8. The measured sensor response time in flow-through configuration is about five times shorter respect to standard flow-over configuration.

Paper Details

Date Published: 2 May 2017
PDF: 1 pages
Proc. SPIE 10081, Frontiers in Biological Detection: From Nanosensors to Systems IX, 100810C (2 May 2017); doi: 10.1117/12.2253612
Show Author Affiliations
Romeo Bernini, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
Immacolata Angelica Grimaldi, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
Gianluca Persichetti, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)
Genni Testa, Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)


Published in SPIE Proceedings Vol. 10081:
Frontiers in Biological Detection: From Nanosensors to Systems IX
Amos Danielli; Benjamin L. Miller; Sharon M. Weiss, Editor(s)

© SPIE. Terms of Use
Back to Top