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Proceedings Paper

Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy
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Paper Abstract

Propagation of action potentials arises on millisecond timescales, suggesting the need for advancement of methods capable of commensurate volume rendering for in vivo brain mapping. In practice, beam-scanning multiphoton microscopy is widely used to probe brain function, striking a balance between simplicity and penetration depth. However, conventional beam-scanning platforms generally do not provide access to full volume renderings at the speeds necessary to map propagation of action potentials. By combining a sparse sampling strategy based on Lissajous trajectory microscopy in combination with temporal multiplexing for simultaneous imaging of multiple focal planes, whole volumes of cells are potentially accessible each millisecond.

Paper Details

Date Published: 22 February 2017
PDF: 6 pages
Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 1007606 (22 February 2017); doi: 10.1117/12.2253514
Show Author Affiliations
Andreas C. Geiger, Purdue Univ. (United States)
Justin A. Newman, Purdue Univ. (United States)
Suhas Sreehari, Purdue Univ. (United States)
Shane Z. Sullivan, Purdue Univ. (United States)
Charles A. Bouman, Purdue Univ. (United States)
Garth J. Simpson, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 10076:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II
Kevin K. Tsia; Keisuke Goda, Editor(s)

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