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Proceedings Paper

Detection of lipopolysaccharides in serum using a waveguide-based optical biosensor
Author(s): Aneesa Noormohamed; Loreen R. Stromberg; Aaron S. Anderson; Zachary Karim; Priya Dighe; Prakasha Kempaiah; John M. Ong'echa; Douglas J. Perkins; Norman Doggett; Benjamin McMahon; Harshini Mukundan
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Paper Abstract

Direct ultra-sensitive detection of pathogen biomarkers in blood could provide a universal strategy for diagnosis of bacterial infections, which remain a leading cause of morbidity and mortality in many areas of the world. Many factors complicate diagnosis, including the presence of multiple co-infections in a given patient, and lack of infrastructure in rural settings. In some pediatric patients, such as those in areas with poor resources, an additional challenge exists with low sample volumes due to age and other health factors such as anemia and dehydration. Our team is working on developing novel diagnostic assays, with a waveguide-based biosensor platform, to rapidly and specifically identify pathogen biomarkers from small samples of serum or plasma, allowing for the timely and sensitive diagnosis of infection at the point of care. In addition to the platform, we have developed novel membrane insertion and lipoprotein capture assay methods, to capture lipidated pathogen biomarkers in aqueous blood, by virtue of their interactions with host lipoprotein carriers. Herein, we demonstrate our efforts to adapt the lipoprotein capture assay for the detection of small concentrations of pathogen-secreted lipopolysaccharides in aqueous blood, with the ultimate aim of diagnosing Gram-negative infections effectively.

Paper Details

Date Published: 17 February 2017
PDF: 8 pages
Proc. SPIE 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics, 100720A (17 February 2017); doi: 10.1117/12.2253506
Show Author Affiliations
Aneesa Noormohamed, Los Alamos National Lab. (United States)
Loreen R. Stromberg, The Univ. of New Mexico (United States)
Aaron S. Anderson, Los Alamos National Lab. (United States)
Zachary Karim, The Univ. of New Mexico (United States)
Priya Dighe, Los Alamos National Lab. (United States)
Prakasha Kempaiah, The Univ. of New Mexico (United States)
John M. Ong'echa, The Univ. of New Mexico/Kenya Medical Research Institute (Kenya)
Douglas J. Perkins, The Univ. of New Mexico (United States)
Norman Doggett, Los Alamos National Lab. (United States)
Benjamin McMahon, Los Alamos National Lab. (United States)
Harshini Mukundan, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 10072:
Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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