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Proceedings Paper

Optimized signal detection and analysis methods for in vivo photoacoustic flow cytometry
Author(s): Qiyan Wang; Quanyu Zhou; Ping Yang; Xiaoling Wang; Zhenyu Niu; Yuanzhen Suo; Hao He; Wenyuan Gao; Shuo Tang; Xunbin Wei
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Paper Abstract

Melanoma is known as a malignant tumor of melanocytes, which usually appear in the blood circulation at the metastasis stage of cancer. Thus the detection of circulating melanoma cells is useful for early diagnosis and therapy of cancer. Here we have developed an in vivo photoacoustic flow cytometry (PAFC) based on the photoacoustic effect to detect melanoma cells. However, the raw signals we obtain from the target cells contain noises such as environmental sonic noises and electronic noises. Therefore we apply correlation comparison and feature separation methods to the detection and verification of the in vivo signals. Due to similar shape and structure of cells, the photoacoustic signals usually have similar vibration mode. By analyzing the correlations and the signal features in time domain and frequency domain, we are able to provide a method for separating photoacoustic signals generated by target cells from background noises. The method introduced here has proved to optimize the signal acquisition and signal processing, which can improve the detection accuracy in PAFC.

Paper Details

Date Published: 20 February 2017
PDF: 8 pages
Proc. SPIE 10065, Biophotonics and Immune Responses XII, 100650C (20 February 2017); doi: 10.1117/12.2253495
Show Author Affiliations
Qiyan Wang, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)
Quanyu Zhou, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)
Ping Yang, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)
Xiaoling Wang, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)
Zhenyu Niu, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)
Yuanzhen Suo, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)
Hao He, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)
Wenyuan Gao, Binzhou Medical Univ. (China)
Shuo Tang, Univ. of British Columbia (Canada)
Xunbin Wei, Med-X Research Institute, Shanghai Jiao Tong Univ. (China)


Published in SPIE Proceedings Vol. 10065:
Biophotonics and Immune Responses XII
Wei R. Chen, Editor(s)

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