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Proceedings Paper

ReagentTF: a rapid and versatile optical clearing method for biological imaging (Conference Presentation)
Author(s): Tingting Yu; Jingtan Zhu; Yusha Li; Yisong Qi; Jianyi Xu; Hui Gong; Qingming Luo; Dan Zhu
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Paper Abstract

The emergence of various optical clearing methods provides a great potential for imaging deep inside tissues by combining with multiple-labelling and microscopic imaging techniques. They were generally developed for specific imaging demand thus presented some non-negligible limitations such as long incubation time, tissue deformation, fluorescence quenching, incompatibility with immunostaining or lipophilic tracers. In this study, we developed a rapid and versatile clearing method, termed ReagentTF, for deep imaging of various fluorescent samples. This method can not only efficiently clear embryos, neonatal whole-brains and adult thick brain sections by simple immersion in aqueous mixtures with minimal volume change, but also can preserve fluorescence of various fluorescent proteins and simultaneously be compatible with immunostaining and lipophilic neuronal dyes. We demonstrate the effectiveness of this method in reconstructing the cell distributions of mouse hippocampus, visualizing the neural projection from CA1 (Cornu Ammonis 1) to HDB (nucleus of the horizontal limb of the diagonal band), and observing the growth of forelimb plexus in whole-mount embryos. These results suggest that ReagentTF is useful for large-volume imaging and will be an option for the deep imaging of biological tissues.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10051, Neural Imaging and Sensing, 100510J (19 April 2017); doi: 10.1117/12.2253491
Show Author Affiliations
Tingting Yu, Huazhong Univ. of Science and Technology (China)
Jingtan Zhu, Huazhong Univ. of Science and Technology (China)
Yusha Li, Huazhong Univ. of Science and Technology (China)
Yisong Qi, Huazhong Univ. of Science and Technology (China)
Jianyi Xu, Huazhong Univ. of Science and Technology (China)
Hui Gong, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Dan Zhu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10051:
Neural Imaging and Sensing
Qingming Luo; Jun Ding, Editor(s)

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