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Proceedings Paper

Scattering angle resolved optical coherence tomography for in vivo murine retinal imaging
Author(s): Michael R. Gardner; Nitesh Katta; Austin McElroy; Vikram Baruah; H. Grady Rylander; Thomas E. Milner
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Paper Abstract

Optical coherence tomography (OCT) retinal imaging contributes to understanding central nervous system (CNS) diseases because the eye is an anatomical “window to the brain” with direct optical access to nonmylenated retinal ganglion cells. However, many CNS diseases are associated with neuronal changes beyond the resolution of standard OCT retinal imaging systems. Though studies have shown the utility of scattering angle resolved (SAR) OCT for particle sizing and detecting disease states ex vivo, a compact SAR-OCT system for in vivo rodent retinal imaging has not previously been reported. We report a fiber-based SAR-OCT system (swept source at 1310 nm ± 65 nm, 100 kHz scan rate) for mouse retinal imaging with a partial glass window (center aperture) for angular discrimination of backscattered light. This design incorporates a dual-axis MEMS mirror conjugate to the ocular pupil plane and a high collection efficiency objective. A muring retina is imaged during euthanasia, and the proposed SAR-index is examined versus time. Results show a positive correlation between the SAR-index and the sub-cellular hypoxic response of neurons to isoflurane overdose during euthanasia. The proposed SAR-OCT design and image process technique offer a contrast mechanism able to detect sub-resolution neuronal changes for murine retinal imaging.

Paper Details

Date Published: 17 February 2017
PDF: 6 pages
Proc. SPIE 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, 100531O (17 February 2017); doi: 10.1117/12.2253367
Show Author Affiliations
Michael R. Gardner, The Univ. of Texas at Austin (United States)
Nitesh Katta, The Univ. of Texas at Austin (United States)
Austin McElroy, The Univ. of Texas at Austin (United States)
Vikram Baruah, The Univ. of Texas at Austin (United States)
H. Grady Rylander, The Univ. of Texas at Austin (United States)
Thomas E. Milner, The Univ. of Texas at Austin (United States)


Published in SPIE Proceedings Vol. 10053:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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