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Proceedings Paper

Optical biopsy of tissue with Mueller polarimetry: theory and experiments (Conference Presentation)
Author(s): Tatiana Novikova; Igor Meglinski; Enric Garcia-Caurel; Alexander Bykov; Jean Rehbinder; Stanislas Deby; Jérémy Vizet; Angelo Pierangelo; François Moreau; Pierre Validire; Abdelali Benali; Brice Gayet; Benjamin Teig; André Nazac; Razvigor Ossikovski

Paper Abstract

The rise of optical biopsy as an alternative to classical biopsy is dictated by ongoing technological progress: any type of measurements has to be fast, precise, non-invasive and implemented in-vivo. The use of polarized light for optical biopsy has a long history. As Mueller-Stokes formalism provides the most complete description of polarized light interaction with any type of sample (even depolarizing one) we explored the capabilities of in-house multi-wavelength Mueller imaging polarimeter for the detection of pre-malignancy and malignancy. Our studies were performed with both scattering phantom tissues (in transmission configuration) and specimens of human colon and uterine cervix (in backscattering configuration). For the interpretation of measurement results we decomposed Mueller matrix of a sample into product of elementary Mueller matrices of homogeneous diattenuator, retarder, and depolarizer. This phenomenological approach does not require the exact solution of Maxwell equations and provides the “effective” values of polarimetric properties of sample. Exploring differential Mueller matrix formalism for fluctuating medium we showed that depolarization in homogeneous turbid medium varied parabolically with the pathlength of transmitted light, while the standard deviation of elementary polarization properties of medium depends linearly on the concentration of scatterers. Neither scattering phantoms nor human tissue possessed any measurable diattenuation in backscattering configuration. The polarimetric images of tissue depolarization power, scalar birefringence and orientation of optical axis were compared with the analysis of histological slides. The spectral dependence of depolarization power and scalar birefringence values ascertained the potential of imaging Mueller polarimetry to discriminate healthy and diseased tissue zones.

Paper Details

Date Published: 1 May 2017
PDF: 1 pages
Proc. SPIE 10060, Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 100600V (1 May 2017); doi: 10.1117/12.2253123
Show Author Affiliations
Tatiana Novikova, Ecole Polytechnique (France)
Igor Meglinski, Univ. of Oulu (Finland)
Enric Garcia-Caurel, Ecole Polytechnique (France)
Alexander Bykov, Univ. of Oulu (Finland)
Jean Rehbinder, Ecole Polytechnique (France)
Stanislas Deby, Ecole Polytechnique (France)
Jérémy Vizet, Ecole Polytechnique (France)
Angelo Pierangelo, Ecole Polytechnique (France)
François Moreau, Ecole Polytechnique (France)
Pierre Validire, Institut Mutualiste Montsouris (France)
Abdelali Benali, Institut Mutualiste Montsouris (France)
Brice Gayet, Institut Mutualiste Montsouris (France)
Benjamin Teig, CHU Bicêtre (France)
André Nazac, CHU Bicêtre (France)
Razvigor Ossikovski, Ecole Polytechnique (France)

Published in SPIE Proceedings Vol. 10060:
Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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