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Proceedings Paper

An infrared optical pacing system for high-throughput screening of cardiac electrophysiology in human cardiomyocytes (Conference Presentation)
Author(s): Matt T. McPheeters; Yves T. Wang; Kenneth R. Laurita; Michael W. Jenkins
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Paper Abstract

Cardiomyocytes derived from human induced pluripotent stem cells (hiPS-HCM) have the potential to provide individualized therapies for patients and to test drug candidates for cardiac toxicity. In order for hiPS-CM to be useful for such applications, there is a need for high-throughput technology to rapidly assess cardiac electrophysiology parameters. Here, we designed and tested a fully contactless optical mapping (OM) and optical pacing (OP) system capable of imaging and point stimulation of hiPS-CM in small wells. OM allowed us to characterize cardiac electrophysiological parameters (conduction velocity, action potential duration, etc.) using voltage-sensitive dyes with high temporal and spatial resolution over the entire well. To improve OM signal-to-noise ratio, we tested a new voltage-sensitive dye (Fluovolt) for accuracy and phototoxicity. Stimulation is essential because most electrophysiological parameters are rate dependent; however, traditional methods utilizing electrical stimulation is difficult in small wells. To overcome this limitation, we utilized OP (λ = 1464 nm) to precisely control heart rate with spatial precision without the addition of exogenous agents. We optimized OP parameters (e.g., well size, pulse width, spot size) to achieve robust pacing and minimize the threshold radiant exposure. Finally, we tested system sensitivity using Flecainide, a drug with well described action on multiple electrophysiological properties.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10042, Diagnostic and Therapeutic Applications of Light in Cardiology, 100420F (19 April 2017); doi: 10.1117/12.2253110
Show Author Affiliations
Matt T. McPheeters, Case Western Reserve Univ. (United States)
Yves T. Wang, Case Western Reserve Univ. (United States)
Kenneth R. Laurita, Case Western Reserve Univ. (United States)
Michael W. Jenkins, Case Western Reserve Univ. (United States)


Published in SPIE Proceedings Vol. 10042:
Diagnostic and Therapeutic Applications of Light in Cardiology
Guillermo J. Tearney; Kenton W. Gregory; Laura Marcu, Editor(s)

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