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Proceedings Paper

A flexible and rapid frequency selective scheme for SRS microscopy
Author(s): Jingting Li; Yuankai Yue; Wei-Chuan Shih
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Paper Abstract

Stimulated Raman scattering (SRS) is a label-free imaging technique suitable for studying biological systems. Due to stimulated nature by ultrafast laser pulses, SRS microscopy has the advantage of significantly higher sensitivity but often reduced spectroscopic information. In this paper, we present a newly constructed femtosecond SRS microscope with a high-speed dynamic micromirror device based pulse shaper to achieve flexible and rapid frequency selection within the C-H stretch region near 2800 to 3100 cm-1 with spectral width of 30 cm-1. This technique is applicable to lipid profiling such as cell activity mapping, lipid distribution mapping and distinction among subclasses.

Paper Details

Date Published: 21 February 2017
PDF: 5 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006914 (21 February 2017); doi: 10.1117/12.2253094
Show Author Affiliations
Jingting Li, Univ. of Houston (United States)
Yuankai Yue, Univ. of Houston (United States)
Wei-Chuan Shih, Univ. of Houston (United States)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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