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Proceedings Paper

Chemotaxis of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy
Author(s): Björn Kemper; Jürgen Schnekenburger; Steffi Ketelhut
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Paper Abstract

We investigated the capabilities of digital holographic microscopy (DHM) for label-free quantification of the response of living single cells to chemical stimuli in 3D assays. Fibro sarcoma cells were observed in a collagen matrix inside 3D chemotaxis chambers with a Mach-Zehnder interferometer-based DHM setup. From the obtained series of quantitative phase images, the migration trajectories of single cells were retrieved by automated cell tracking and subsequently analyzed for maximum migration distance and motility. Our results demonstrate DHM as a highly reliable and efficient tool for label-free quantification of chemotaxis in 2D and 3D environments.

Paper Details

Date Published: 21 February 2017
PDF: 6 pages
Proc. SPIE 10074, Quantitative Phase Imaging III, 1007412 (21 February 2017); doi: 10.1117/12.2253091
Show Author Affiliations
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)
Jürgen Schnekenburger, Westfälische Wilhelms-Univ. Münster (Germany)
Steffi Ketelhut, Westfälische Wilhelms-Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 10074:
Quantitative Phase Imaging III
Gabriel Popescu; YongKeun Park, Editor(s)

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