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Proceedings Paper

Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models
Author(s): Gwendolyn M. Cramer; Hamid El-Hamidi; Jonathan P. Celli
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Paper Abstract

Pancreatic ductal adenocarcinoma (PDAC) is characterized by extracellular matrix-rich stromal involvement, but it is not clear how ECM properties that affect invasiveness and chemotherapy response influence efficacy of photodynamic therapy (PDT). To disentangle the mechanical and biochemical effects of ECM composition, we measured the effects of various combinations of ECM proteins on growth behavior, invasive potential, and therapeutic response of multicellular 3D pancreatic tumor models. These spheroids were grown in attachment-free conditions before embedding in combinations of rheologically characterized collagen 1 and Matrigel combinations and treated with oxaliplatin chemotherapy and PDT. We find that cells invading from collagen-embedded tumor spheroids, the least rigid ECM substrate described here, displayed better response to PDT than to oxaliplatin chemotherapy. Overall, our results support that ECM-mediated invading PDAC populations remain responsive to PDT in conditions that induce chemoresistance.

Paper Details

Date Published: 8 February 2017
PDF: 7 pages
Proc. SPIE 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, 100470O (8 February 2017); doi: 10.1117/12.2252983
Show Author Affiliations
Gwendolyn M. Cramer, Univ. of Massachusetts Boston (United States)
Hamid El-Hamidi, Univ. of Massachusetts Boston (United States)
Jonathan P. Celli, Univ. of Massachusetts Boston (United States)


Published in SPIE Proceedings Vol. 10047:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI
David H. Kessel; Tayyaba Hasan, Editor(s)

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