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Proceedings Paper

Phototodynamic activity of zinc monocarboxyphenoxy phthalocyane (ZnMCPPc) conjugated to gold silver (AuAg) nanoparticles in melanoma cancer cells
Author(s): Sello Lebohang Manoto; David Oluyinka Oluwole; Rudzani Malabi; Charles Maphanga; Saturnin Ombinda-Lemboumba; Tebello Nyokong; Patience Mthunzi-Kufa
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Paper Abstract

Photodynamic therapy (PDT) is a minimally invasive therapeutic modality for the treatment of neoplastic and non-neoplastic diseases. In PDT of cancer, irradiation with light of a specific wavelength leads to activation of a photosensitizer which results in generation of reactive oxygen species (ROS) which induces cell death. Many phthalocyanine photosensitizers are hydrophobic and insoluble in water, which limits their therapeutic efficiency. Consequently, advanced delivery systems and strategies are needed to improve the effectiveness of these photosensitizers. Nanoparticles have shown promising results in increasing aqueous solubility, bioavailability, stability and delivery of photosensitizers to their target. This study investigated the photodynamic activity of zinc monocarboxyphenoxy phthalocyanine (ZnMCPPc) conjugated to gold silver (AuAg) nanoparticles in melanoma cancer cells. The photodynamic activity of ZnMCPPc conjugated to AuAg nanoparticles were evaluated using cellular morphology, viability, proliferation and cytotoxicity. Untreated cells showed no changes in cellular morphology, proliferation and cytotoxicity. However, photoactivated ZnMCPPc conjugated to AuAg nanoparticles showed changes in cell morphology and a dose dependent decrease in cellular viability, proliferation and an increase in cell membrane damage. The ZnMCPPc conjugated to AuAg nanoparticles used in this study was highly effective in inducing cell death of melanoma cancer cells.

Paper Details

Date Published: 8 February 2017
PDF: 11 pages
Proc. SPIE 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, 1004716 (8 February 2017); doi: 10.1117/12.2252838
Show Author Affiliations
Sello Lebohang Manoto, CSIR National Laser Ctr. (South Africa)
David Oluyinka Oluwole, Rhodes Univ. (South Africa)
Rudzani Malabi, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)
Charles Maphanga, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)
Saturnin Ombinda-Lemboumba, CSIR National Laser Ctr. (South Africa)
Tebello Nyokong, Rhodes Univ. (South Africa)
Patience Mthunzi-Kufa, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)


Published in SPIE Proceedings Vol. 10047:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI
David H. Kessel; Tayyaba Hasan, Editor(s)

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