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Preservation media analysis for ex vivo measurements of endogenous UV fluorescence of liver fibrosis in bulk samples
Author(s): Enoch Gutierrez-Herrera; Adolfo Perez-Garcia; Nathalie Aleman-García; Antonio Ortega-Martinez; Celia Sánchez-Pérez; Walfre Franco; Joselín Hernández-Ruiz
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Paper Abstract

Non-subjective, minimally-invasive, and quantifying techniques may support development and evaluation of a fibrosis regression treatment. The build-up of extracellular matrix in liver fibrosis may result on changes of the endogenous fluorescence of tissue. In this work, we evaluate the fluorescence excitation/emission matrix in the UV range for several bulk samples of murine hepatic tissue preserved in different media. Chemical changes on tissue, caused by formaldehyde preservation, alter the endogenous fluorescence spectra. To avoid these drawbacks, phosphate-buffered saline (PBS) or Iscove’s Modified Dulbecco’s Medium were used. PBS buffer showed to be the less harmful and cost-effective preservation medium to study the endogenous fluorescence in fibrotic tissue.

Paper Details

Date Published: 15 February 2017
PDF: 7 pages
Proc. SPIE 10062, Optical Interactions with Tissue and Cells XXVIII, 100621C (15 February 2017); doi: 10.1117/12.2252795
Show Author Affiliations
Enoch Gutierrez-Herrera, Univ. Nacional Autónoma de México (Mexico)
Adolfo Perez-Garcia, Hospital General de México Dr. Eduardo Liceaga (Mexico)
Nathalie Aleman-García, Univ. Nacional Autónoma de Mexico (Mexico)
Antonio Ortega-Martinez, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard School of Medicine (United States)
Celia Sánchez-Pérez, Univ. Nacional Autónoma de México (Mexico)
Walfre Franco, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard School of Medicine (United States)
Joselín Hernández-Ruiz, Hospital General de México Dr. Eduardo Liceaga (Mexico)


Published in SPIE Proceedings Vol. 10062:
Optical Interactions with Tissue and Cells XXVIII
E. Duco Jansen; Hope Thomas Beier, Editor(s)

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