Share Email Print
cover

Proceedings Paper

High-speed amplitude modulation of femtosecond laser pulses for frequency-multiplexed two photon imaging
Author(s): Dmitri Tsyboulski; Natalia Orlova; Peter Saggau
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We present a frequency-multiplexed multi-site two-photon imaging method utilizing amplitude modulation of femtosecond laser pulses in the MHz range to tag each excitation beam and the corresponding fluorescence signals with specific frequencies. The frequency tags are generated with an interferometric setup employing acousto-optic deflectors (AODs) to achieve precise spatial overlap of femtosecond pulses with acoustically-shifted frequencies. Creating matching excitation beam patterns in each interferometer arm using multiple AOD driving frequencies and subsequently overlapping these matching patterns results in multiple excitation beams with unique beat frequencies available for scanning. As a proof-of-concept, we demonstrate multiplexed two-photon image acquisition using test samples, and compare the performance of this approach to conventional two-photon laser scanning microscopy.

Paper Details

Date Published: 21 February 2017
PDF: 8 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691T (21 February 2017); doi: 10.1117/12.2252627
Show Author Affiliations
Dmitri Tsyboulski, Allen Institute for Brain Science (United States)
Natalia Orlova, Allen Institute for Brain Science (United States)
Peter Saggau, Allen Institute for Brain Science (United States)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

© SPIE. Terms of Use
Back to Top