Share Email Print
cover

Proceedings Paper

The effects of prolonged oral administration of gold nanoparticles on the morphology of hematopoietic and lymphoid organs
Author(s): Alla B. Bucharskaya; Svetlana S. Pakhomy; Olga V. Zlobina; Galina N. Maslyakova; Nikita A. Navolokin; Olga V. Matveeva; Boris N. Khlebtsov; Vladimir A. Bogatyrev; Nikolai G. Khlebtsov; Valery V. Tuchin
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Currently, the usage of gold nanoparticles as photosensitizers and immunomodulators for plasmonic photothermal therapy has attracted a great attention of researches and end-users. In our work, the influence of prolonged peroral administration of gold nanoparticles (GNPs) with different sizes on the morphological changes of hematopoietic and lymphoid organs was investigated. The 24 white outbred male rats weighing 180-220 g were randomly divided into groups and administered orally for 30 days the suspension of gold nanospheres with diameters of 2, 15 and 50 nm at a dosage of 190 μg/kg of animal body weight. To prevent GNPs aggregation in a tissue and enhance biocompatibility, they were functionalized with thiolated polyethylene glycol. The withdrawal of the animals from the experiment and sampling of spleen, lymph nodes and bone marrow tissues for morphological study were performed a day after the last administration. In the spleen the boundary between the red and white pulp was not clearly differ in all experimental groups, lymphoid follicles were significantly increased in size, containing bright germinative centers represented by large blast cells. The stimulation of lymphocyte and myelocytic series of hematopoiesis was recorded at morphological study of the bone marrow. The number of immunoblasts and large lymphocytes was increased in all structural zones of lymph nodes. The more pronounced changes were found in the group with administration of 15 nm nanoparticles. Thus, the morphological changes of cellular components of hematopoietic organs have size-dependent character and indicate the activation of the migration, proliferation and differentiation of immune cells after prolonged oral administration of GNPs.

Paper Details

Date Published: 20 February 2017
PDF: 7 pages
Proc. SPIE 10065, Biophotonics and Immune Responses XII, 100650V (20 February 2017); doi: 10.1117/12.2252534
Show Author Affiliations
Alla B. Bucharskaya, Saratov State Medical Univ. (Russian Federation)
Svetlana S. Pakhomy, Saratov State Medical Univ. (Russian Federation)
Olga V. Zlobina, Saratov State Medical Univ. (Russian Federation)
Galina N. Maslyakova, Saratov State Medical Univ. (Russian Federation)
Nikita A. Navolokin, Saratov State Medical Univ. (Russian Federation)
Olga V. Matveeva, Saratov State Medical Univ. (Russian Federation)
Boris N. Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Vladimir A. Bogatyrev, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Nikolai G. Khlebtsov, Saratov National Research State Univ. (Russian Federation)
Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Valery V. Tuchin, Saratov National Research State Univ. (Russian Federation)
Institute of Precision Mechanics and Control (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 10065:
Biophotonics and Immune Responses XII
Wei R. Chen, Editor(s)

© SPIE. Terms of Use
Back to Top