Share Email Print
cover

Proceedings Paper

Orthotopic AY-27 rat bladder urothelial cell carcinoma model presented an elevated methemoglobin proportion in the increased total hemoglobin content when evaluated in vivo by single-fiber reflectance spectroscopy
Author(s): Tengfei Sun; Carole A. Davis; Robert E. Hurst; Joel W. Slaton; Daqing Piao
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In vivo single-fiber reflectance spectroscopy (SfRS) was performed on an orthotopic AY-27 rat bladder urothelial cell carcinoma model to explore potential spectroscopic features revealing neoplastic changes. AY-27 bladder tumor cells were intravesically instilled in four rats and allowed to implant and grow for one week, with two additional rats as the control. A total of 107 SfRS measurements were taken from 27 sites on two control bladders and 80 from four AY-27 treated bladders. The spectral profiles obtained from AY-27 treated bladders revealed various levels of a methemoglobin (MetHb) characteristic spectral feature around 635nm. A multisegment spectral analysis method estimated concentrations of five chromophore compositions including oxyhemoglobin, deoxyhemoglobin, MetHb, lipid and water. The total hemoglobin concentration ([HbT]), the MetHb proportion in the total hemoglobin and the lipid volume content showed possible correlations. The 80 measurements from the AY-27 treated bladders could separate to three sub-sets according to the MetHb proportion. Specifically, 72 were in subset 1 with low proportion (5.3%<[MetHb]<7%), 6 in subset 2 with moderate proportion (7%<[MetHb]<30%), and 2 in subset 3 with significant proportion (>30%). When grouped according to [MetHB], the [HbT] increased from 368 μM of subset 1 to 488 μM of subset 2 to 541 μM of subset 3, in comparison to the 285 μM of the control. The increased total hemoglobin and the elevation of MetHb proportion may signify angiogenesis and degradation in hemoglobin oxygen-transport. Additionally, the lipid volume content decreased from 2.58% in the control to <0.2% in the tumor groups, indicating disruption of subepithelium tissue architecture.

Paper Details

Date Published: 6 February 2017
PDF: 12 pages
Proc. SPIE 10038, Therapeutics and Diagnostics in Urology: Lasers, Robotics, Minimally Invasive, and Advanced Biomedical Devices, 100380L (6 February 2017); doi: 10.1117/12.2252419
Show Author Affiliations
Tengfei Sun, Oklahoma State Univ. (United States)
Carole A. Davis, The Univ. of Oklahoma Health Sciences Ctr. (United States)
Robert E. Hurst, The Univ. of Oklahoma Health Sciences Ctr. (United States)
Joel W. Slaton, The Univ. of Oklahoma Health Sciences Ctr. (United States)
Daqing Piao, Oklahoma State Univ. (United States)


Published in SPIE Proceedings Vol. 10038:
Therapeutics and Diagnostics in Urology: Lasers, Robotics, Minimally Invasive, and Advanced Biomedical Devices
Hyun Wook Kang; Kin Foong Chan, Editor(s)

Video Presentation

Orthotopic-AY-27-rat-bladder-urothelial-cell-carcinoma-model-presented



© SPIE. Terms of Use
Back to Top