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Proceedings Paper

Genetically encoded sensors and fluorescence microscopy for anticancer research
Author(s): Elena V. Zagaynova; Marina V. Shirmanova; Tatiana F. Sergeeva; Natalia V. Klementieva; Alexander S. Mishin; Alena I. Gavrina; Olga A. Zlobovskay; Olga E. Furman; Varvara V. Dudenkova; Gregory S. Perelman; Maria M. Lukina; Konstantin A. Lukyanov
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Paper Abstract

Early response of cancer cells to chemical compounds and chemotherapeutic drugs were studied using novel fluorescence tools and microscopy techniques. We applied confocal microscopy, two-photon fluorescence lifetime imaging microscopy and super-resolution localization-based microscopy to assess structural and functional changes in cancer cells in vitro. The dynamics of energy metabolism, intracellular pH, caspase-3 activation during staurosporine-induced apoptosis as well as actin cytoskeleton rearrangements under chemotherapy were evaluated. We have showed that new genetically encoded sensors and advanced fluorescence microscopy methods provide an efficient way for multiparameter analysis of cell activities

Paper Details

Date Published: 21 February 2017
PDF: 8 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691K (21 February 2017); doi: 10.1117/12.2252151
Show Author Affiliations
Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)
Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)
Tatiana F. Sergeeva, Nizhny Novgorod State Medical Academy (Russian Federation)
Natalia V. Klementieva, Nizhny Novgorod State Medical Academy (Russian Federation)
Alexander S. Mishin, Nizhny Novgorod State Medical Academy (Russian Federation)
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Alena I. Gavrina, Nizhny Novgorod State Medical Academy (Russian Federation)
Olga A. Zlobovskay, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS (Russian Federation)
Olga E. Furman, Nizhny Novgorod State Medical Academy (Russian Federation)
Varvara V. Dudenkova, Nizhny Novgorod State Medical Academy (Russian Federation)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Gregory S. Perelman, Nizhny Novgorod State Medical Academy (Russian Federation)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Maria M. Lukina, Nizhny Novgorod State Medical Academy (Russian Federation)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Konstantin A. Lukyanov, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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