Share Email Print
cover

Proceedings Paper

In vivo metabolic imaging of mouse tumor models in response to chemotherapy
Author(s): Maria M. Lukina; Varvara Dudenkova; Anastasia V. Shumilova; Ludmila B. Snopova; Elena V. Zagaynova; Marina V. Shirmanova
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

The aim of the study was to estimate energy metabolism in human cervical cancer cells HeLa Kyoto after chemotherapy in vitro and in vivo using two-photon fluorescence lifetime microscopy (FLIM). Cellular metabolism was examined by monitoring of the fluorescence intensities and lifetimes of metabolic cofactors NAD(P)H and FAD. Cancer metabolism was analyzed in dynamics after treatment with cisplatin. Two-photon fluorescence and second harmonic generation microscopies as well as standard histopathology with hematoxylin and eosin were used to characterize cancer tissue structure. We showed an increase of the optical redox ratio FAD/NAD(P)H in cancer cells in vitro and decrease of the relative contribution of free NAD(P)H (ɑ1) in vitro and in vivo, which presumably indicate a shift to more oxidative metabolism after chemotherapy. These data demonstrate the possibility to detect response of cancer cells to chemotherapy using optical metabolic imaging.

Paper Details

Date Published: 2 March 2017
PDF: 8 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692G (2 March 2017); doi: 10.1117/12.2252123
Show Author Affiliations
Maria M. Lukina, Nizhny Novgorod State Medical Academy (Russian Federation)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Varvara Dudenkova, Nizhny Novgorod State Medical Academy (Russian Federation)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Anastasia V. Shumilova, Nizhny Novgorod State Medical Academy (Russian Federation)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Ludmila B. Snopova, Nizhny Novgorod State Medical Academy (Russian Federation)
Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)
Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

© SPIE. Terms of Use
Back to Top