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Proceedings Paper

Multimodal nonlinear optical imaging of cartilage development in mouse model
Author(s): Sicong He; Wenqian Xue; Qiqi Sun; Xuesong Li; Jiandong Huang; Jianan Y. Qu
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Paper Abstract

Kinesin-1 is a kind of motor protein responsible for intracellular transportation and has been studied in a variety of tissues. However, its roles in cartilage development are not clear. In this study, a kinesin-1 heavy chain (Kif5b) knockout mouse model is used to study the functions of kinesin-1 in the cartilage development. We developed a multimodal nonlinear optical (NLO) microscope system integrating stimulated Raman scattering (SRS), second harmonic generation (SHG) and two-photon excited fluorescence (TPEF) to investigate the morphological and biomedical characteristics of fresh tibial cartilage from normal and mutant mice at different developmental stages. The combined forward and backward SHG imaging resolved the fine structure of collagen fibrils in the extracellular matrix of cartilage. Meanwhile, the chondrocyte morphology in different zones of cartilage was visualized by label-free SRS and TPEF images. The results show that the fibrillar collagen in the superficial zone of cartilage in postnatal day 10 and 15 (P10 and P15) knockout mice was significantly less than that of control mice. Moreover, we observed distorted morphology and disorganization of columnar arrangement of chondrocytes in the growth plate cartilage of mutant mice. This study reveals the significant roles of kinesin-1 in collagen formation and chondrocyte morphogenesis.

Paper Details

Date Published: 21 February 2017
PDF: 7 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690O (21 February 2017); doi: 10.1117/12.2252066
Show Author Affiliations
Sicong He, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Wenqian Xue, The Univ. of Hong Kong (Hong Kong, China)
Qiqi Sun, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Xuesong Li, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jiandong Huang, The Univ. of Hong Kong (China)
HKU-Shenzhen Institute of Research and Innovation (China)
Shenzhen Institute of Advanced Technologies (China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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