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Proceedings Paper

Optical cell stimulation for neuronal excitation (Conference Presentation)
Author(s): Sonja Johannsmeier; Patrick Heeger; Mitsuhiro Terakawa; Alexander Heisterkamp; Tammo Ripken; Dag Heinemann

Paper Abstract

Optical manipulation of cellular functions represents a growing field in biomedical sciences. The possibility to modulate specific targets with high spatial and temporal precision in a contactless manner allows a broad range of applications. Here, we present a study on stimulation of neuronal cells by optical means. As a long-term objective, we seek to improve the performance of current electric neurostimulation, especially in the context of cochlear implants. Firstly, we tested a gold nanoparticle mediated approach to modulate transmembrane conductivity by irradiation using a picosecond pulsed Nd:YAG laser at 532 nm for 40 ms in a neuroblastoma cell line (N2A) and primary murine neurons. The light absorption leads to a rapid temperature increase of the gold nanoparticles, which can induce an increased permeabilisation of the cellular membrane. Calcium transients were recorded as an indicator of neuronal activity. Although calcium signals were reliably detected upon laser irradiation, the temporal behavior did not resemble action potentials. The origin of these signals was investigated by an inhibitor study. These results indicate calcium induced calcium release (CICR) as the major source of the calcium transients. Consecutively, we tested alternative approaches for cell stimulation, such as glutamate release and optogenetics, and evaluated the potential of these methods for the application in a cochlear implant. Compared to the gold nanoparticle approach, both techniques induce less cellular stress and reliably produce action potentials.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10052, Optogenetics and Optical Manipulation, 100520E (19 April 2017); doi: 10.1117/12.2252017
Show Author Affiliations
Sonja Johannsmeier, Laser Zentrum Hannover e.V. (Germany)
NIFE - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development (Germany)
Patrick Heeger, Leibniz Univ. Hannover (Germany)
NIFE - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development (Germany)
Mitsuhiro Terakawa, Keio Univ. (Japan)
Alexander Heisterkamp, Leibniz Univ. Hannover (Germany)
NIFE - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development (Germany)
Tammo Ripken, Laser Zentrum Hannover e.V. (Germany)
Dag Heinemann, Laser Zentrum Hannover e.V. (Germany)
NIFE - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development (Germany)


Published in SPIE Proceedings Vol. 10052:
Optogenetics and Optical Manipulation
Samarendra K. Mohanty; Nitish V. Thakor; E. Duco Jansen, Editor(s)

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