Share Email Print
cover

Proceedings Paper

Fast quantitative retardance imaging of biological samples using quandri-wave interferometry (Conference Presentation)
Author(s): Sherazade Aknoun; Julien Savatier; Pierre Bon; Benoit Wattellier; Serge Monneret

Paper Abstract

We describe a technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear birefringence measurements on biological samples [1] such as living cells and tissues. The system combines QPI with different excitation polarizations to create retardance images. This creates a new kind of image contrast based on the local retardance, reveals the structure of sample anisotropic components and adds specificity to label-free phase images. We implemented this technique allowing us to take retardance images in less than 1 second which allows us to make high speed acquisitions to reconstruct tissues virtual slides with different modalities (i.e intensity, phase and retardance). Comparisons between healthy and tumoral 10 µm thick skin tissues and collagen orientation studies in the latter will be presented. [1] S. Aknoun, P. Bon, J. Savatier, B. Wattellier, and S. Monneret, "Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry," Opt. Express 23, 16383-16406 (2015).

Paper Details

Date Published: 24 April 2017
PDF: 1 pages
Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 1006802 (24 April 2017); doi: 10.1117/12.2251975
Show Author Affiliations
Sherazade Aknoun, PHASICS S.A. (France)
Julien Savatier, Institut Fresnel, UMR 7249 (France)
Aix-Marseille Univ. (France)
CNRS (France)
Pierre Bon, Institut d'Optique Graduate School (France)
Bordeaux Univ. (France)
CNRS (France)
Benoit Wattellier, PHASICS S.A. (France)
Serge Monneret, Institut Fresnel, UMR 7249 (France)
Aix-Marseille Univ. (France)
CNRS (France)


Published in SPIE Proceedings Vol. 10068:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top