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Proceedings Paper

Multiple speckle illumination for optical-resolution photoacoustic imaging
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Paper Abstract

Optical-resolution photoacoustic microscopy offers exquisite and specific contrast to optical absorption. Conventional approaches generally involves raster scanning a focused spot over the sample. Here, we demonstrate that a full-field illumination approach with multiple speckle illumination can also provide diffraction-limited optical-resolution photoacoustic images. Two different proof-of-concepts are demonstrated with micro-structured test samples. The first approach follows the principle of correlation/ghost imaging,1, 2 and is based on cross-correlating photoacoustic signals under multiple speckle illumination with known speckle patterns measured during a calibration step. The second approach is a speckle scanning microscopy technique, which adapts the technique proposed in fluorescence microscopy by Bertolotti and al.:3 in our work, spatially unresolved photoacoustic measurements are performed for various translations of unknown speckle patterns. A phase-retrieval algorithm is used to reconstruct the object from the knowledge of the modulus of its Fourier Transform yielded by the measurements. Because speckle patterns naturally appear in many various situations, including propagation through biological tissue or multi-mode fibers (for which focusing light is either very demanding if not impossible), speckle-illumination-based photoacoustic microscopy provides a powerful framework for the development of novel reconstruction approaches, well-suited to compressed sensing approaches.2

Paper Details

Date Published: 3 March 2017
PDF: 5 pages
Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 100641O (3 March 2017); doi: 10.1117/12.2251909
Show Author Affiliations
Florian Poisson, Univ. Grenoble Alpes, CNRS, Lab. Interdisciplinaire de Physique (France)
ESPCI ParisTech, PSL Research Univ., CNRS, INSERM, Univ. Paris-Diderot, Institut Langevin (France)
Nicolino Stasio, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Christophe Moser, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Demetri Psaltis, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Emmanuel Bossy, Univ. Grenoble Alpes, CNRS, Lab. Interdisciplinaire de Physique (France)
ESPCI ParisTech, PSL Research Univ., CNRS, INSERM, Univ. Paris-Diderot, Institut Langevin (France)
École Polytechnique Fédérale de Lausanne (France)


Published in SPIE Proceedings Vol. 10064:
Photons Plus Ultrasound: Imaging and Sensing 2017
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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