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Proceedings Paper

Brain vascular image segmentation based on fuzzy local information C-means clustering
Author(s): Chaoen Hu; Xia Liu; Xiao Liang; Hui Hui; Xin Yang; Jie Tian
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Paper Abstract

Light sheet fluorescence microscopy (LSFM) is a powerful optical resolution fluorescence microscopy technique which enables to observe the mouse brain vascular network in cellular resolution. However, micro-vessel structures are intensity inhomogeneity in LSFM images, which make an inconvenience for extracting line structures. In this work, we developed a vascular image segmentation method by enhancing vessel details which should be useful for estimating statistics like micro-vessel density. Since the eigenvalues of hessian matrix and its sign describes different geometric structure in images, which enable to construct vascular similarity function and enhance line signals, the main idea of our method is to cluster the pixel values of the enhanced image. Our method contained three steps: 1) calculate the multiscale gradients and the differences between eigenvalues of Hessian matrix. 2) In order to generate the enhanced microvessels structures, a feed forward neural network was trained by 2.26 million pixels for dealing with the correlations between multi-scale gradients and the differences between eigenvalues. 3) The fuzzy local information c-means clustering (FLICM) was used to cluster the pixel values in enhance line signals. To verify the feasibility and effectiveness of this method, mouse brain vascular images have been acquired by a commercial light-sheet microscope in our lab. The experiment of the segmentation method showed that dice similarity coefficient can reach up to 85%. The results illustrated that our approach extracting line structures of blood vessels dramatically improves the vascular image and enable to accurately extract blood vessels in LSFM images.

Paper Details

Date Published: 16 February 2017
PDF: 5 pages
Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100680Q (16 February 2017); doi: 10.1117/12.2251725
Show Author Affiliations
Chaoen Hu, Harbin Univ. of Science and Technology (China)
Institute of Chinese Academy of Sciences (China)
Institute of Automation (China)
Xia Liu, Harbin Univ. of Science and Technology (China)
Xiao Liang, Institute of Chinese Academy of Sciences (China)
Institute of Automation (China)
Hui Hui, Institute of Chinese Academy of Sciences (China)
Institute of Automation (China)
Xin Yang, Institute of Chinese Academy of Sciences (China)
Institute of Automation (China)
Jie Tian, Institute of Chinese Academy of Sciences (China)
Institute of Automation (China)


Published in SPIE Proceedings Vol. 10068:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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