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Proceedings Paper

Imaging of single retinal ganglion cell with differential interference contrast microscopy (Conference Presentation)
Author(s): Juyeong Oh; Yu Jeong Kim; Chul-Ki Kim; Taik Jin Lee; Mina Seo; Seok Lee; Deok Ha Woo; Seong Chan Jun; Ki-Ho Park; Seok Hwan Kim; Jae Hun Kim
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Paper Abstract

Glaucoma is a progressive optic neuropathy, characterized by the selective loss of retinal ganglion cells (RGCs). Therefore, monitoring the change of number or morphology of RGC is essential for the early detection as well as investigation of pathophysiology of glaucoma. Since RGC layer is transparent and hyporeflective, the direct optical visualization of RGCs has not been successful so far. Therefore, glaucoma evaluation mostly depends on indirect diagnostic methods such as the evaluation of optic disc morphology or retinal nerve fiber layer thickness measurement by optical coherence tomography. We have previously demonstrated single photoreceptor cell imaging with differential interference contrast (DIC) microscopy. Herein, we successfully visualized single RGC using DIC microscopy. Since RGC layer is much less reflective than photoreceptor layer, various techniques including the control of light wavelength and bandwidth using a tunable band pass filter were adopted to reduce the chromatic aberration in z-axis for higher and clearer resolution. To verify that the imaged cells were the RGCs, the flat-mounted retina of Sprague-Dawley rat, in which the RGCs were retrogradely labeled with fluorescence, was observed by both fluorescence and DIC microscopies for direct comparison. We have confirmed that the cell images obtained by fluorescence microscopy were perfectly matched with cell images by DIC microscopy. As conclusion, we have visualized single RGC with DIC microscopy, and confirmed with fluorescence microscopy.

Paper Details

Date Published: 16 May 2017
PDF: 1 pages
Proc. SPIE 10045, Ophthalmic Technologies XXVII, 100450N (16 May 2017); doi: 10.1117/12.2251585
Show Author Affiliations
Juyeong Oh, Korea Institute of Science and Technology (Korea, Republic of)
Yonsei Univ. (Korea, Republic of)
Yu Jeong Kim, Seoul National Univ. Hospital (Korea, Republic of)
Chul-Ki Kim, Korea Institute of Science and Technology (Korea, Republic of)
Taik Jin Lee, Korea Institute of Science and Technology (Korea, Republic of)
Mina Seo, Korea Institute of Science and Technology (Korea, Republic of)
Seok Lee, Korea Institute of Science and Technology (Korea, Republic of)
Deok Ha Woo, Korea Institute of Science and Technology (Korea, Republic of)
Seong Chan Jun, Yonsei Univ. (Korea, Republic of)
Ki-Ho Park, Seoul National Univ. Boramae Hospital (Korea, Republic of)
Seok Hwan Kim, Seoul National Univ. Boramae Hospital (Korea, Republic of)
Jae Hun Kim, Korea Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 10045:
Ophthalmic Technologies XXVII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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