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Proceedings Paper

Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy
Author(s): Franziska Foertsch; Mykhailo Ilchenko; Thomas Heitkamp; Silke Noßmann; Birgit Hoffmann; Ilka Starke; Ralf Mrowka; Christoph Biskup; Michael Börsch
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Paper Abstract

Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enzymes are located in the inner mitochondrial membrane (IMM), which exhibits large invaginations called cristae. According to recent electron cryotomography, FoF1-ATP synthases are located predominantly at the rim of the cristae, while cytochrome C oxidases are likely distributed in planar membrane areas of the cristae. Previous FLIM measurements (K. Busch and coworkers) of complex II and III unravelled differences in the local environment of the membrane enzymes in the cristae. Here, we tagged complex IV and V with mNeonGreen and investigated their mitochondrial nano-environment by FLIM and superresolution microscopy in living human cells. Different lifetimes and anisotropy values were found and will be discussed.

Paper Details

Date Published: 21 February 2017
PDF: 14 pages
Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710P (21 February 2017); doi: 10.1117/12.2251210
Show Author Affiliations
Franziska Foertsch, Universitätsklinikum Jena (Germany)
Mykhailo Ilchenko, Universitätsklinikum Jena (Germany)
Thomas Heitkamp, Universitätsklinikum Jena (Germany)
Silke Noßmann, Universitätsklinikum Jena (Germany)
Birgit Hoffmann, Universitätsklinikum Jena (Germany)
Ilka Starke, Universitätsklinikum Jena (Germany)
Ralf Mrowka, Universitätsklinikum Jena (Germany)
Ctr. for Medical Optics and Photonics (Germany)
Christoph Biskup, Universitätsklinikum Jena (Germany)
Ctr. for Medical Optics and Photonics (Germany)
Michael Börsch, Universitätsklinikum Jena (Germany)
Ctr. for Medical Optics and Photonics (Germany)


Published in SPIE Proceedings Vol. 10071:
Single Molecule Spectroscopy and Superresolution Imaging X
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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