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Proceedings Paper

Ultrafast, laser-scanning time-stretch microscopy with visible light
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Paper Abstract

We demonstrate ultrafast time-stretch microscopy in, to the best of our knowledge, the shortest wavelength regimes, i.e. 532 nm. This is enabled by a new all-optical ultrahigh-speed laser-scanning technique called free-space angular-chirpenhanced delay (FACED) that achieves a line-scan rate as high as 20 MHz. In contrast to the predominant fiber-based implementation, time-stretch imaging based on FACED allows wavelength-independent and low-loss operations, and more intriguingly reconfigurable all-optical laser-scanning rate. Using this technique, we present high-resolution single-cell images captured in an ultrafast microfluidic flow (1.5m/s). This could unleash numerous cell and tissue imaging applications, e.g. high-throughput image flow cytometry and whole-slide imaging.

Paper Details

Date Published: 16 February 2017
PDF: 6 pages
Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100681C (16 February 2017); doi: 10.1117/12.2251094
Show Author Affiliations
Wenwei Yan, The Univ. of Hong Kong (Hong Kong, China)
Jianglai Wu, The Univ. of Hong Kong (Hong Kong, China)
Kenneth K. Y. Wong, The Univ. of Hong Kong (Hong Kong, China)
Kevin K. Tsia, The Univ. of Hong Kong (Hong Kong, China)


Published in SPIE Proceedings Vol. 10068:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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