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Proceedings Paper

A pH-sensitive red fluorescent protein compatible with hydrophobic resin embedding
Author(s): Wenyan Guo; Yadong Gang; Xiuli Liu; Hongfu Zhou; Shaoqun Zeng
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Paper Abstract

pH sensitive fluorescent proteins enabling chemical reactivation in resin are useful tools for fluorescence microimaging. EYFP or EGFP improved from GFP in jellyfish are good for such applications. For simultaneous two-color imaging, a suitable red fluorescent protein is of urgent need. Here a pH sensitive red fluorescent protein, pHuji, is selected and verified to be compatible with hydrophobic resin embedding and thus may be promising for dual-colour chemical reactivation imaging in conjunction with EGFP or EYFP.

Paper Details

Date Published: 8 February 2017
PDF: 4 pages
Proc. SPIE 10051, Neural Imaging and Sensing, 1005119 (8 February 2017); doi: 10.1117/12.2250844
Show Author Affiliations
Wenyan Guo, Huazhong Univ. of Science and Technology (China)
Yadong Gang, Huazhong Univ. of Science and Technology (China)
Xiuli Liu, Huazhong Univ. of Science and Technology (China)
Hongfu Zhou, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10051:
Neural Imaging and Sensing
Qingming Luo; Jun Ding, Editor(s)

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