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Proceedings Paper

The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging
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Paper Abstract

We have simulated the effects of the number of bits and the sampling rate of a digitizer on the performance of lifetime measurements. We found that the number of bits of a digitizer is important to obtain certain accuracy in lifetime measurement. There exists a certain critical sampling frequency of a digitizer required to separate a certain lifetime differences in a double exponentially decaying intensity profile. We did these simulations by using Monte Carlo simulations with least-square curve fitting algorithms.

Paper Details

Date Published: 22 February 2017
PDF: 5 pages
Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 100761H (22 February 2017); doi: 10.1117/12.2250730
Show Author Affiliations
Won Sang Hwang, Yonsei Univ. (Korea, Republic of)
Dong Eun Kim, Yonsei Univ. (Korea, Republic of)
Jun Woo Kim, Yonsei Univ. (Korea, Republic of)
Youn Young Ji, Yonsei Univ. (Korea, Republic of)
Byung Hwy So, Yonsei Univ. (Korea, Republic of)
Dug Young Kim, Yonsei Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 10076:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II
Kevin K. Tsia; Keisuke Goda, Editor(s)

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