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Proceedings Paper

Hybrid ultrasound and dual-wavelength optoacoustic biomicroscopy for functional neuroimaging
Author(s): Johannes Rebling; Hector Estrada; Michael Zwack; Gali Sela; Sven Gottschalk; Daniel Razansky
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Paper Abstract

Many neurological disorders are linked to abnormal activation or pathological alterations of the vasculature in the affected brain region. Obtaining simultaneous morphological and physiological information of neurovasculature is very challenging due to the acoustic distortions and intense light scattering by the skull and brain. In addition, the size of cerebral vasculature in murine brains spans an extended range from just a few microns up to about a millimeter, all to be recorded in 3D and over an area of several dozens of mm2. Numerous imaging techniques exist that excel at characterizing certain aspects of this complex network but are only capable of providing information on a limited spatiotemporal scale. We present a hybrid ultrasound and dual-wavelength optoacoustic microscope, capable of rapid imaging of murine neurovasculature in-vivo, with high spatial resolution down to 12 μm over a large field of view exceeding 50mm2. The dual wavelength imaging capability allows for the visualization of functional blood parameters through an intact skull while pulse-echo ultrasound biomicroscopy images are captured simultaneously by the same scan head. The flexible hybrid design in combination with fast high-resolution imaging in 3D holds promise for generating better insights into the architecture and function of the neurovascular system.

Paper Details

Date Published: 3 March 2017
PDF: 6 pages
Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 100644T (3 March 2017); doi: 10.1117/12.2250635
Show Author Affiliations
Johannes Rebling, Institute for Biological and Medical Imaging, Helmholtz Zentrum München GmbH (Germany)
Technical Univ. of Munich (Germany)
Hector Estrada, Institute for Biological and Medical Imaging, Helmholtz Zentrum München GmbH (Germany)
Michael Zwack, Technical Univ. of Munich (Germany)
Gali Sela, Institute for Biological and Medical Imaging, Helmholtz Zentrum München GmbH (Germany)
Sven Gottschalk, Institute for Biological and Medical Imaging, Helmholtz Zentrum München GmbH (Germany)
Daniel Razansky, Institute for Biological and Medical Imaging, Helmholtz Zentrum München GmbH (Germany)
Technical Univ. of Munich (Germany)


Published in SPIE Proceedings Vol. 10064:
Photons Plus Ultrasound: Imaging and Sensing 2017
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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