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Proceedings Paper

Wide-field fluorescence diffuse optical tomography with epi-illumination of sinusoidal pattern
Author(s): Tongxin Li; Feng Gao; Weiting Chen; Caixia Qi; Panpan Yan; Huijuan Zhao
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Paper Abstract

We present a wide-field fluorescence tomography with epi-illumination of sinusoidal pattern. In this scheme, a DMD projector is employed as a spatial light modulator to generate independently wide-field sinusoidal illumination patterns at varying spatial frequencies on a sample, and then the emitted photons at the sample surface were captured with a EM-CCD camera. This method results in a significantly reduced number of the optical field measurements as compared to the point-source-scanning ones and thereby achieves a fast data acquisition that is desired for a dynamic imaging application. Fluorescence yield images are reconstructed using the normalized-Born formulated inversion of the diffusion model. Experimental reconstructions are presented on a phantom embedding the fluorescent targets and compared for a combination of the multiply frequencies. The results validate the ability of the method to determine the target relative depth and quantification with an increasing accuracy.

Paper Details

Date Published: 17 February 2017
PDF: 6 pages
Proc. SPIE 10059, Optical Tomography and Spectroscopy of Tissue XII, 100591Y (17 February 2017); doi: 10.1117/12.2250474
Show Author Affiliations
Tongxin Li, Tianjin Univ. (China)
Feng Gao, Tianjin Univ. (China)
Tianjin Key Lab. of Biomedical Detecting Techniques and Instruments (China)
Weiting Chen, Tianjin Univ. (China)
Caixia Qi, Tianjin Univ. (China)
Panpan Yan, Tianjin Univ. (China)
Huijuan Zhao, Tianjin Univ. (China)
Tianjin Key Lab. of Biomedical Detecting Techniques and Instruments (China)


Published in SPIE Proceedings Vol. 10059:
Optical Tomography and Spectroscopy of Tissue XII
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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