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Proceedings Paper

Photoacoustic imaging of intestinal strictures: microscopic and macroscopic assessment in vivo (Conference Presentation)
Author(s): Guan Xu; Hao Lei; Laura A. Johnson; David S. Moons; Teng Ma; Qifa Zhou; Michael D. Rice; Jun Ni; Xueding Wang; Peter D. R. Higgins

Paper Abstract

The pathology of Crohn’s disease (CD) is characterized by obstructing intestinal strictures because of inflammation (with high levels of hemoglobin), fibrosis (high levels of collagen), or a combination of both. Inflammatory strictures are medically treated. Fibrotic strictures have to be removed surgically. The accurate characterization of the strictures is therefore critical for the management of CD. Currently the comprehensive assessment of a stricture is difficult, as the standard diagnostic procedure, endoscopic biopsy, is superficial and with limited locations as well as depth. In our previous studies, photoacoustic imaging (PAI) has recovered the layered architectures and the relative content of the molecular components in human and animal tissues ex vivo. This study will investigate the capability of multispectral PAI in resolving the architecture and the molecular components of intestinal strictures in rats in vivo. PA images at 532, 1210 and 1310 nm targeting the strong optical absorption of hemoglobin, lipid and collagen were acquired using two approaches. A compact linear array, CL15-7, was used to transcutaneously acquire PA signals generated by the a fiber optics diffuser positioned within the inner lumen of the strictures. Another approach was to use an endoscopic capsule probe for acoustic resolution PA microscopy. The capsule probe is designed for human and therefore cannot fit into rat colon. The inner surface of the intestinal stricture was exposed and the probe was attached to the diseased location for imaging. The findings in PA images were confirmed by histology results.

Paper Details

Date Published: 24 April 2017
PDF: 1 pages
Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 100642B (24 April 2017); doi: 10.1117/12.2250424
Show Author Affiliations
Guan Xu, Univ. of Michigan Medical School (United States)
Hao Lei, Univ. of Michigan (United States)
Laura A. Johnson, Univ. of Michigan Medical School (United States)
David S. Moons, Univ. of Michigan Medical School (United States)
Teng Ma, The Univ. of Southern California (United States)
Qifa Zhou, The Univ. of Southern California (United States)
Michael D. Rice, Univ. of Michigan Medical School (United States)
Jun Ni, Univ. of Michigan (United States)
Xueding Wang, Univ. of Michigan Medical School (United States)
Peter D. R. Higgins, Univ. of Michigan Medical School (United States)


Published in SPIE Proceedings Vol. 10064:
Photons Plus Ultrasound: Imaging and Sensing 2017
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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