Share Email Print
cover

Proceedings Paper

New sensorless wavefront estimation approach for two-photon scanning microscopy
Author(s): J. Teixeira; D. Champelovier; J-M. Conan; L. Mugnier; S. Meimon; N. Balla; S. Reichinnek; T. Tressard; R. Cossart; S. Monneret; H. Rigneault; A. Malvache
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

The landscape of biomedical research in neuroscience has changed dramatically in recent years as a result of spectacular progress in dynamic microscopy. In this context Adaptive Optics allows in-depth imaging by correcting aberrations induced by the biological sample, the key issue being then the ability to perform an accurate and reliable wavefront sensing (WFS). We present here a limitation of modal sensorless WFS in the case of a heterogeneous medium. We then build a new method called Axially-Locked Modal Sensorless (ALMS) that exploits these heterogeneities to overcome this limitation. The new method is simulated and compared to standard modal sensorless. The simulation results show a more accurate wavefront estimation even in the case of a strongly aberrated biological media.

Paper Details

Date Published: 21 February 2017
PDF: 7 pages
Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 1007317 (21 February 2017); doi: 10.1117/12.2250323
Show Author Affiliations
J. Teixeira, ONERA - the French Aerospace Lab. (France)
D. Champelovier, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
INMED, INSERM, Aix-Marseille Univ. (France)
J-M. Conan, ONERA - the French Aerospace Lab. (France)
L. Mugnier, ONERA - the French Aerospace Lab. (France)
S. Meimon, ONERA - the French Aerospace Lab. (France)
N. Balla, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
S. Reichinnek, INMED, INSERM, Aix-Marseille Univ. (France)
T. Tressard, INMED, INSERM, Aix-Marseille Univ. (France)
R. Cossart, INMED, INSERM, Aix-Marseille Univ. (France)
S. Monneret, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
H. Rigneault, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
A. Malvache, Institut Fresnel, Aix-Marseille Univ., CNRS (France)
INMED, INSERM, Aix-Marseille Univ. (France)


Published in SPIE Proceedings Vol. 10073:
Adaptive Optics and Wavefront Control for Biological Systems III
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

© SPIE. Terms of Use
Back to Top