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Proceedings Paper

In vivo multiphoton microscopy beyond 1 mm in the brain
Author(s): David R. Miller; Flor A. Medina; Ahmed Hassan; Evan P. Perillo; Kristen Hagan; S. M. Shams Kazmi; Boris V. Zemelman; Andrew K. Dunn
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Paper Abstract

We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between λ=1,100 and 1,400 nm. We demonstrate an imaging depth of 1,200 μm in vasculature and 1,160 μm in neurons. We also demonstrate deep-tissue imaging using Indocyanine Green (ICG), which is FDA approved and a promising route to translate multiphoton microscopy to human applications.

Paper Details

Date Published: 21 February 2017
PDF: 7 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690C (21 February 2017); doi: 10.1117/12.2249949
Show Author Affiliations
David R. Miller, The Univ. of Texas at Austin (United States)
Flor A. Medina, The Univ. of Texas at Austin (United States)
Ahmed Hassan, The Univ. of Texas at Austin (United States)
Evan P. Perillo, The Univ. of Texas at Austin (United States)
Kristen Hagan, The Univ. of Texas at Austin (United States)
S. M. Shams Kazmi, The Univ. of Texas at Austin (United States)
Boris V. Zemelman, The Univ. of Texas at Austin (United States)
Andrew K. Dunn, The Univ. of Texas at Austin (United States)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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