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Proceedings Paper

Label-free imaging of cortical structures with multiphoton microscopy
Author(s): Shu Wang; Xiuqiang Chen; Weilin Wu; Zhida Chen; Ruolan Lin; Peihua Lin; Xingfu Wang; Yu Vincent Fu; Jianxin Chen
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Paper Abstract

Cortical structures in the central nervous system exhibit an ordered laminar organization. Defined cell layers are significant to our understanding of brain structure and function. In this work, multiphoton microscopy (MPM) based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), which was applied for qualitatively visualizing the structure of cerebral and cerebellar cortex from the fresh, unfixed, and unstained specimen. MPM is able to effectively identify neurons and neurites in cerebral cortex, as well as glial cells, Purkinje cells, and granule cells in cerebellar cortex at subcellular resolution. In addition, the use of automated image processing algorithms can quantify the circularity of neurons and the density distribution of neurites based on the intrinsic nonlinear optical contrast, further providing quantitative characteristics for automatically analyzing the laminar structure of cortical structures. These results suggest that with the development of the feasibility of two-photon fiberscopes and microendoscope probes, the combined MPM and image analysis holds potential to provide supplementary information to augment the diagnostic accuracy of neuropathology and in vivo identification of various neurological illnesses in clinic.

Paper Details

Date Published: 2 March 2017
PDF: 9 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692J (2 March 2017); doi: 10.1117/12.2249920
Show Author Affiliations
Shu Wang, Fujian Normal Univ. (China)
Xiuqiang Chen, Institute of Microbiology (China)
Weilin Wu, Fujian Normal Univ. (China)
Zhida Chen, Fujian Normal Univ. (China)
Ruolan Lin, Fujian Medical Univ. (China)
Peihua Lin, Fujian Normal Univ. (China)
Xingfu Wang, Fujian Medical Univ. (China)
Yu Vincent Fu, Institute of Microbiology (China)
Jianxin Chen, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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