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Proceedings Paper

Local pressure and matrix component effects on verteporfin distribution in pancreatic tumors (Conference Presentation)
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Paper Abstract

Pancreatic tumors are characterized by large interstitial hypertension from enhanced deposition of extracellular matrix components, resulting in widespread vascular collapse and reduced molecular uptake of systemically delivered therapies. Although the origins of hypoperfusion is debated amongst researchers, spatial distribution of collagen density and hyaluronic acid content have shown to be a key metric in understanding the lack of efficacy for both acute and chronic therapies in these tumors. In this study, the AsPC-1 tumor model was used both subcutaneously and orthotopically to study the measurable factors which are related to this. A conventional piezoelectric pressure catheter was used to measure total tissue pressure (TTP), defined as a combination of solid stress (SS) and interstitial fluid pressure (IFP), TTP = SS + IFP, in multiple locations within the tumor interstitium. Matrix components such as collagen and hyaluronic acid were scored using masson’s trichrome stain and hyaluronic acid binding protein (HABP), respectively, and co-registered with values of TTP. The results show that these key measurements are related to the spatial distribution of verteporfin in the same tumors. Photodynamic treatment with verteporfin is known to ablate large regions of tumor tissue and also allow better permeability for chemotherapies. The study of spatial distribution of verteporfin in relation to stromal content and TTP will help us better control these types of combination therapies.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, 1004708 (19 April 2017); doi: 10.1117/12.2249766
Show Author Affiliations
Michael D. Nieskoski, Thayer School of Engineering at Dartmouth (United States)
Kayla Marra, Thayer School of Engineering at Dartmouth (United States)
Jason R. Gunn, Thayer School of Engineering at Dartmouth (United States)
Marvin Doyley, Univ. of Rochester (United States)
Kimberly S. Samkoe, Thayer School of Engineering at Dartmouth (United States)
Stephen P. Pereira, Univ. College London (United Kingdom)
B. Stuart Trembly, Thayer School of Engineering at Dartmouth (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)


Published in SPIE Proceedings Vol. 10047:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI
David H. Kessel; Tayyaba Hasan, Editor(s)

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