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Proceedings Paper

Femtosecond two-photon absorption spectra and permanent electric dipole moment change of tryptophan, 2-aminopurine and related intrinsic and synthetic fluorophores
Author(s): Alexander Mikhaylov; Jake R. Lindquist; Patrik R. Callis; Bern Kohler; Juri Pahapill; Sophie de Reguardati; Matt Rammo; Merle Uudsemaa; Aleksander Trummal; Aleksander Rebane
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Paper Abstract

Two-photon absorption (2PA) cross sections for 2-aminopurine (2AP), 7-methyl guanosine (7MG), 6-methyl isoxanthopterin (6MI), isoxanthopterin (IXP), 3-methylindole (3MI) and tryptophan (L-trp) are measured for excitation wavelengths between 550 and 810 nm using two independent methods: femtosecond two-photon excited fluorescence (2PEF) and femtosecond nonlinear transmission (NLT). For all systems we find that in the long wavelength region of the lowest-energy one-photon allowed singlet-singlet transition the 2PA spectral profiles follow the one-photon absorption spectral profiles, thus indicating the strongly dipolar nature of the transition. We use the measured 2PA cross sections to estimate the corresponding permanent electric dipole moment change and find the values to vary in the range 1 to 5 D. These experimental findings are corroborated by quantum-chemical computations.

Paper Details

Date Published: 21 February 2017
PDF: 11 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006920 (21 February 2017); doi: 10.1117/12.2249634
Show Author Affiliations
Alexander Mikhaylov, Montana State Univ. (United States)
Jake R. Lindquist, Montana State Univ. (United States)
Patrik R. Callis, Montana State Univ. (United States)
Bern Kohler, Montana State Univ. (United States)
The Ohio State Univ. (United States)
Juri Pahapill, National Institute of Chemical Physics and Biophysics (Estonia)
Sophie de Reguardati, National Institute of Chemical Physics and Biophysics (Estonia)
Matt Rammo, National Institute of Chemical Physics and Biophysics (Estonia)
Merle Uudsemaa, National Institute of Chemical Physics and Biophysics (Estonia)
Aleksander Trummal, National Institute of Chemical Physics and Biophysics (Estonia)
Aleksander Rebane, Montana State Univ. (United States)
National Institute of Chemical Physics and Biophysics (Estonia)


Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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