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Proceedings Paper

Research on detecting plankton based on darkfield microscopy
Author(s): Buyu Guo; Jia Yu; Huiping Liu; Xiaoying Liu; Rui Hou
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Paper Abstract

Plankton is an important part of the marine life, which plays a significant role in studying the change of global climate and the marine ecological environment. In order to detect plankton accurately and effectively, we design the Underwater In-situ Dark-field Microscopy Detection System based on the principle of dark-field microscopy. The experiment results show that while the 2× microscope objective is used, the resolution is 90.5lp/mm and the horizontal field of view is 2.6×2.6mm and while the 10× microscope objective is used, the resolution is 181lp/mm and the horizontal field of view is 2.2×2.2mm, when the microscope objective is replaced by 0.9× large format telecentric lens, the detection area will be 11mm with the resolution of 32lp/mm. Double compartment structure is designed, such that the system can work underwater.

Paper Details

Date Published: 31 October 2016
PDF: 7 pages
Proc. SPIE 10021, Optical Design and Testing VII, 100211M (31 October 2016); doi: 10.1117/12.2246238
Show Author Affiliations
Buyu Guo, Ocean Univ. of China (China)
Jia Yu, Ocean Univ. of China (China)
Huiping Liu, Ocean Univ. of China (China)
Xiaoying Liu, Ocean Univ. of China (China)
Rui Hou, Ocean Univ. of China (China)


Published in SPIE Proceedings Vol. 10021:
Optical Design and Testing VII
Yongtian Wang; Tina E. Kidger; Kimio Tatsuno, Editor(s)

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