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Proceedings Paper

Detection and classification of ebola on microfluidic chips
Author(s): Xue Lin; Xiangyu Jin; Yunqian Fan; Qin Huang; Yue Kou; Guo Zu; Shiguang Huang; Xiaosheng Liu; Guoliang Huang
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Paper Abstract

Point-of-care testing (POCT) for an infectious diseases is the prerequisite to control of the disease and limitation of its spread. A microfluidic chip for detection and classification of four strains of Ebola virus was developed and evaluated. This assay was based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and specific primers for Ebola Zaire virus, Ebola Sudan virus, Ebola Tai Forest virus and Ebola Bundibugyo virus were designed. The sensitivity of the microfluidic chip was under 103 copies per milliliter, as determined by ten repeated tests. This assay is unique in its ability to enable diagnosis of the Ebola infections and simultaneous typing of Ebola virus on a single chip. It offers short reaction time, ease of use and high specificity. These features should enable POCT in remote area during outbreaks of Ebola virus.

Paper Details

Date Published: 31 October 2016
PDF: 6 pages
Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100240B (31 October 2016); doi: 10.1117/12.2246154
Show Author Affiliations
Xue Lin, Tsinghua Univ. School of Medicine (China)
Xiangyu Jin, Tsinghua Univ. School of Medicine (China)
Yunqian Fan, Tsinghua Univ. School of Medicine (China)
Qin Huang, National Engineering Research Ctr. for Beijing Biochip Technology (China)
Yue Kou, National Engineering Research Ctr. for Beijing Biochip Technology (China)
Guo Zu, Tsinghua Univ. School of Medicine (China)
Shiguang Huang, Capital Medical Univ. (China)
Xiaosheng Liu, Tsinghua Univ. School of Medicine (China)
Guoliang Huang, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)


Published in SPIE Proceedings Vol. 10024:
Optics in Health Care and Biomedical Optics VII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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