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Proceedings Paper

Quantitative optical biomarkers of lung cancer based intrinsic two-photon excited fluorescence signal
Author(s): Jingwen Li; Zhenlin Zhan; Hongxin Lin; Ning Zuo; Xiaoqin Zhu; Shusen Xie; Jianxin Chen; Shuangmu Zhuo
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Paper Abstract

Alterations in the elastic fibers have been implicated in lung cancer. However, the label-free, microscopic imaging of elastic fibers in situ remains a major challenge. Here, we present the use of intrinsic two-photon excited fluorescence (TPEF) signal as a novel means for quantification of the elastic fibers in intact fresh human lung tissues. We obtained the TPEF images of elastic fibers from ex vivo the human lung tissues. We found that three features, including the elastic fibers area, the elastic fibers orientation, the elastic fibers structure, provide the quantitative identification of lung cancer and the direct visual cues for cancer versus non-cancer areas. These results suggest that the TPEF signal can be used as the label-free optical biomarkers for rapid clinical lung diagnosis and instant image-guided surgery.

Paper Details

Date Published: 31 October 2016
PDF: 8 pages
Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100242I (31 October 2016); doi: 10.1117/12.2245730
Show Author Affiliations
Jingwen Li, Fujian Normal Univ. (China)
Zhenlin Zhan, Fujian Normal Univ. (China)
Hongxin Lin, Fujian Normal Univ. (China)
Ning Zuo, Fujian Normal Univ. (China)
Xiaoqin Zhu, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)
Shuangmu Zhuo, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 10024:
Optics in Health Care and Biomedical Optics VII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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