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Proceedings Paper

Detection of mast cell secretion by using surface enhanced Raman scattering
Author(s): Juan Li; Ren Li; Liqin Zheng; Yuhua Wang; Shusen Xie; Juqiang Lin
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Paper Abstract

Acupuncture can cause a remarkable increase in degranulation of the mast cells, which has attracted the interest of researchers since the 1980s. Surface-enhanced Raman scattering (SERS) could obtain biochemical information with high sensitivity and specificity. In this study, SERS was used to detect the degree of degranulation of mast cells according to different incubate time. Mast cells was incubated with culture medium for 0 h, 12 h and 24 h, then centrifuge the culture medium, decant the supernatant, and discard the mast cell. SERS was performed to obtain the biochemical fingerprinting signatures of the centrifuged medium. The spectra data are then analyzed by spectral peaks attribution and the principal component analysis (PCA). The measured Raman spectra of the two groups were separated well by PCA. It indicated that mast cells had secreted some substances into cultured medium though degranulation did not happen.

Paper Details

Date Published: 31 October 2016
PDF: 4 pages
Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002429 (31 October 2016); doi: 10.1117/12.2245580
Show Author Affiliations
Juan Li, Fujian Normal Univ. (China)
Ren Li, Fujian Normal Univ. (China)
Liqin Zheng, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Juqiang Lin, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 10024:
Optics in Health Care and Biomedical Optics VII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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