Share Email Print
cover

Proceedings Paper

Fluorescence enhancement of photoswitchable metal ion sensors
Author(s): Georgina Sylvia; Sabrina Heng; Andrew D. Abell
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Spiropyran-based fluorescence sensors are an ideal target for intracellular metal ion sensing, due to their biocompatibility, red emission frequency and photo-controlled reversible analyte binding for continuous signal monitoring. However, increasing the brightness of spiropyran-based sensors would extend their sensing capability for live-cell imaging. In this work we look to enhance the fluorescence of spiropyran-based sensors, by incorporating an additional fluorophore into the sensor design. We report a 5-membered monoazacrown bearing spiropyran with metal ion specificity, modified to incorporate the pyrene fluorophore. The effect of N-indole pyrene modification on the behavior of the spiropyran molecule is explored, with absorbance and fluorescence emission characterization. This first generation sensor provides an insight into fluorescence-enhancement of spiropyran molecules.

Paper Details

Date Published: 9 December 2016
PDF: 9 pages
Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100131X (9 December 2016); doi: 10.1117/12.2244656
Show Author Affiliations
Georgina Sylvia, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
The Univ. of Adelaide (Australia)
Sabrina Heng, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
The Univ. of Adelaide (Australia)
Andrew D. Abell, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
The Univ. of Adelaide (Australia)


Published in SPIE Proceedings Vol. 10013:
SPIE BioPhotonics Australasia
Mark R. Hutchinson; Ewa M. Goldys, Editor(s)

© SPIE. Terms of Use
Back to Top