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Proceedings Paper

Label-free assessment of endothelial cell metabolic state using autofluorescent microscopy
Author(s): Benjamin J. Pullen; Tam Nguyen; Martin Gosnell; Ayad G. Anwer; Ewa Goldys; Stephen J. Nicholls; Peter J. Psaltis
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Paper Abstract

To examine the process of endothelial cell aging we utilised hyperspectral imaging to collect broad autofluorescence emission at the individual cellular level and mathematically isolate the characteristic spectra of nicotinamide and flavin adenine dinucleotides (NADH and FAD, respectively). Quantitative analysis of this data provides the basis for a non-destructive spatial imaging method for cells and tissue. FAD and NADH are important factors in cellular metabolism and have been shown to be involved with the redox state of the cell; with the ratio between the two providing the basis for an ‘optical redox ratio’.

Paper Details

Date Published: 9 December 2016
PDF: 1 pages
Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100133F (9 December 2016); doi: 10.1117/12.2244642
Show Author Affiliations
Benjamin J. Pullen, ARC Ctr. of Excellence for Advanced Molecular Imaging (Australia)
The Univ. of Adelaide (Australia)
South Australian Health and Medical Research Institute (Australia)
Tam Nguyen, Univ. of Adelaide (Australia)
Martin Gosnell, The Univ. of Adelaide, (Australia)
Ayad G. Anwer, Ctr. for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Ewa Goldys, Ctr. for Nanoscale BioPhotonics (Australia)
Macquarie Univ. (Australia)
Stephen J. Nicholls, Ctr. for Nanoscale BioPhotonics (Australia)
The Univ. of Adelaide (Australia)
South Australian Health and Medical Research Institute (Australia)
Peter J. Psaltis, The Univ. of Adelaide (Australia)
South Australian Health and Medical Research Institute (Australia)


Published in SPIE Proceedings Vol. 10013:
SPIE BioPhotonics Australasia
Mark R. Hutchinson; Ewa M. Goldys, Editor(s)

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