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Proceedings Paper

Microsphere microscopic imaging with the coherent light
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Paper Abstract

The microsphere has great potential to improve the resolution of the system. The high frequency information of the object can be collected by the microsphere to enhance the imaging resolution. However, the unavoidable chromatic dispersion exists in the microsphere incoherence imaging, which reduces the image quality. In this paper, the microsphere microscopy system is designed with the coherent light. The polystyrene (PS) microspheres with the diameter of 50μm and 90μm are applied, and their refractive index is 1.59. The object is a transmission grating with a cycle of 1.2μm and line spacing of 600 nm. The result indicates that the grating can be clearly detected, and the magnification of the microsphere with the diameter of 50μm and 90μm is 2.33 and 1.96 respectively. Comparing with the traditional white light microscopy, the imaging contrast has been improved though the speckle noise is introduced for coherent light. Therefore the microsphere has the potential to improve the resolution of the phase-contrast imaging.

Paper Details

Date Published: 31 October 2016
PDF: 6 pages
Proc. SPIE 10022, Holography, Diffractive Optics, and Applications VII, 100221N (31 October 2016); doi: 10.1117/12.2243290
Show Author Affiliations
Sha Guo, Beijing Univ. of Technology (China)
Yunxin Wang, Beijing Univ. of Technology (China)
Dayong Wang, Beijing Univ. of Technology (China)
Qiaowen Lin, Beijing Univ. of Technology (China)
Lu Rong, Beijing Univ. of Technology (China)


Published in SPIE Proceedings Vol. 10022:
Holography, Diffractive Optics, and Applications VII
Yunlong Sheng; Chongxiu Yu; Changhe Zhou, Editor(s)

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