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Proceedings Paper

PScan 1.0: flexible software framework for polygon based multiphoton microscopy
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Paper Abstract

Multiphoton laser scanning microscopes exhibit highly localized nonlinear optical excitation and are powerful instruments for in-vivo deep tissue imaging. Customized multiphoton microscopy has a significantly superior performance for in-vivo imaging because of precise control over the scanning and detection system. To date, there have been several flexible software platforms catered to custom built microscopy systems i.e. ScanImage, HelioScan, MicroManager, that perform at imaging speeds of 30-100fps. In this paper, we describe a flexible software framework for high speed imaging systems capable of operating from 5 fps to 1600 fps. The software is based on the MATLAB image processing toolbox. It has the capability to communicate directly with a high performing imaging card (Matrox Solios eA/XA), thus retaining high speed acquisition. The program is also designed to communicate with LabVIEW and Fiji for instrument control and image processing. Pscan 1.0 can handle high imaging rates and contains sufficient flexibility for users to adapt to their high speed imaging systems.

Paper Details

Date Published: 9 December 2016
PDF: 6 pages
Proc. SPIE 10013, SPIE BioPhotonics Australasia, 1001333 (9 December 2016); doi: 10.1117/12.2242954
Show Author Affiliations
Yongxiao Li, The Australian National Univ. (Australia)
Woei Ming Lee, The Australian National Univ. (Australia)


Published in SPIE Proceedings Vol. 10013:
SPIE BioPhotonics Australasia
Mark R. Hutchinson; Ewa M. Goldys, Editor(s)

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