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Proceedings Paper

High speed multiphoton imaging
Author(s): Yongxiao Li; Anne Brustle; Vini Gautam; Ian Cockburn; Cathy Gillespie; Katharina Gaus; Woei Ming Lee
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Paper Abstract

Intravital multiphoton microscopy has emerged as a powerful technique to visualize cellular processes in-vivo. Real time processes revealed through live imaging provided many opportunities to capture cellular activities in living animals. The typical parameters that determine the performance of multiphoton microscopy are speed, field of view, 3D imaging and imaging depth; many of these are important to achieving data from in-vivo. Here, we provide a full exposition of the flexible polygon mirror based high speed laser scanning multiphoton imaging system, PCI-6110 card (National Instruments) and high speed analog frame grabber card (Matrox Solios eA/XA), which allows for rapid adjustments between frame rates i.e. 5 Hz to 50 Hz with 512 × 512 pixels. Furthermore, a motion correction algorithm is also used to mitigate motion artifacts. A customized control software called Pscan 1.0 is developed for the system. This is then followed by calibration of the imaging performance of the system and a series of quantitative in-vitro and in-vivo imaging in neuronal tissues and mice.

Paper Details

Date Published: 9 December 2016
PDF: 5 pages
Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100130K (9 December 2016); doi: 10.1117/12.2242951
Show Author Affiliations
Yongxiao Li, The Australian National Univ. (Australia)
Anne Brustle, The Australian National Univ. (Australia)
Vini Gautam, The Australian National Univ. (Australia)
Ian Cockburn, The Australian National Univ. (Australia)
Cathy Gillespie, The Australian National Univ. (Australia)
Katharina Gaus, The Univ. of New South Wales (Australia)
Woei Ming Lee, The Australian National Univ. (Australia)

Published in SPIE Proceedings Vol. 10013:
SPIE BioPhotonics Australasia
Mark R. Hutchinson; Ewa M. Goldys, Editor(s)

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