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Proceedings Paper

Monitoring of microbial communities by flow cytometry and rRNA-targeted hybridization probes
Author(s): Guenter Wallner; Rudolf Amann
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Paper Abstract

Flow cytometry in combination with ribosomal RNA (rRNA) based fluorescence in situ hybridization is a new technique for the analysis of microbial communities. Oligonucleotide probes directed against ribosomal RNA allow the identification of species or groups of micro- organisms. Combined with flow cytometry, up to several thousand cells per second can be classified. In addition to the identification and specific enumeration of micro-organisms, further information on the distribution of cell size, DNA and ribosome content -- and therefore an assessment of activity -- within the entire community or subpopulations can be obtained. This technique is much more accurate, informative, and rapid than classical culture-dependent methods. Data of activated sludge samples hybridized with fluorescein labeled oligonucleotides and counterstained with the DNA-specific dye Hoechst 33342 are presented as examples for its applicability to complex microbial communities.

Paper Details

Date Published: 9 October 1995
PDF: 4 pages
Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); doi: 10.1117/12.224148
Show Author Affiliations
Guenter Wallner, GSF--Forschungszentrum fuer Umwelt und Gesundheit GmbH and Technische Univ. Muenchen (Germany)
Rudolf Amann, GSF--Forschungszentrum fuer Umwelt und Gesundheit GmbH and Technische Univ. Muenchen (Germany)


Published in SPIE Proceedings Vol. 2504:
Environmental Monitoring and Hazardous Waste Site Remediation
Tuan Vo-Dinh, Editor(s)

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