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Proceedings Paper

Comparison of immunochemical and HPLC-determination of polycyclic aromatic hydrocarbons in groundwater samples of a former manufactured gas plant site
Author(s): Dietmar Knopp; Virpi Vaananen; Reinhard Niessner
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Paper Abstract

Groundwater contamination and PAH-removal with a special water purification facility were monitored over several months by a PAH-immunoassay as well as by HPLC. In the enzyme- linked immunosorbent assay (ELISA) a polyclonal rabbit antiserum was applied. As test format an indirect competitive microtiter plate assay was used. The water samples could be directly measured without any extraction and purification steps. With the ELISA, the limit value for the sum of PAHs of 0.2 ppb as set by the German Drinking Water Act was certainly reached. Including 114 samples no false negative (defined as a measured concentration below 0.2 ppb) but 20 (17.5%) false positive (defined as a measured concentration above 0.2 ppb) samples were found with the immunoassay. Supposing the immunochemical results to be approximate values of the total PAH concentration then these data correlate very well (r equals 0.82, n equals 114) with the sum of the 16 EPA PAHs as measured by HPLC in this study. Therefore, the ELISA could be used as a rapid screening technique to diminish the sample number for HPLC.

Paper Details

Date Published: 9 October 1995
PDF: 8 pages
Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); doi: 10.1117/12.224144
Show Author Affiliations
Dietmar Knopp, Technische Univ. Muenchen (Germany)
Virpi Vaananen, Technische Univ. Muenchen (Germany)
Reinhard Niessner, Technische Univ. Muenchen (Germany)


Published in SPIE Proceedings Vol. 2504:
Environmental Monitoring and Hazardous Waste Site Remediation

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