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Proceedings Paper

Using dual-laser flow cytometry for monitoring phytoplankton composition and integrity
Author(s): Harald Schaefer; Wolfgang Beisker; Christian Steinberg
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Paper Abstract

Dual laser flow cytometry can be used for determining phytoplankton populations in lakes and lowland rivers and streams. Apart from answering basic limnological questions such as the time course of algal blooms or the annual succession of phytoplankton composition further investigations can be made for estimating the integrity of a phytoplankton community using biomass distribution spectra. Thus anthropogenic influence such as eutrophication, acidification or effects of xenobiotica can be monitored. Dual laser flow cytometry with excitation wavelengths of 458 and 528 nm was used to measure photosynthesis pigment fluorescence (chlorophyll a (CHLa), Em greater than 665 nm) and phycoerythrin (PE, Em 575 nm) and cell density of phytoplankton organisms in water samples. CHLa is excited directly by 458 nm and by energy transfer from carotenoids (Ex 528 nm). The ratio of the two fluorescence parameters (CFR) allows users to identify pigment groups in the phytoplankton population (chlorophytes and euglenophytes from chrysophytes, diatoms and dinophytes). PE- containing cyanophytes and cryptophytes can be detected by their PE fluorescence (Ex 528 nm). As a result of preliminary studies for preparing biomass spectra of phytoplankton communities measurements of protein content by staining with fluorescein isothiocyanate (FITC, Ex 488 nm, Em 530 nm) are also shown.

Paper Details

Date Published: 9 October 1995
PDF: 6 pages
Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); doi: 10.1117/12.224133
Show Author Affiliations
Harald Schaefer, GSF-Forschungszentrum fuer Umwelt und Gesundheit GmbH (Germany)
Wolfgang Beisker, GSF-Forschungszentrum fuer Umwelt und Gesundheit (Germany)
Christian Steinberg, Institut fuer Gewaesseroekologie and Binnenfischerei (Germany)

Published in SPIE Proceedings Vol. 2504:
Environmental Monitoring and Hazardous Waste Site Remediation
Tuan Vo-Dinh, Editor(s)

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